Research Paper Volume 13, Issue 2 pp 2626—2639

LncRNA CYTOR affects the proliferation, cell cycle and apoptosis of hepatocellular carcinoma cells by regulating the miR-125b-5p/KIAA1522 axis

MiR-125b-5p directly targets KIAA1522. (A) The differentially expressed mRNAs in HCC were predicted by TCGA database. (B) The intersection of mRNAs combined with miR-125b-5p was shown by Venn diagram. (C) Expression of KIAA1522 and PODXL was increased in HCC tissues compared with normal tissues. (D) Impact of expression of KIAA1522 and PODXL on overall survival in HCC patients in TCGA cohort. (E) miR-125b-5p expression was negatively correlated with KIAA1522 expression. (F) KIAA1522 expression was increased in HCC tissues compared with normal tissues. (G) KIAA1522 expression in HCC tissues and adjacent tissues was detected by immunohistochemistry. (H) KIAA1522 mRNA expression in HCC cells and HHL-5 cells was analyzed by RT-qPCR analysis. ***PI) The binding site between miR-125b-5p and KIAA1522 was predicted by ENCORI. (J) Luciferase reporter assay for the confirmation of direct binding relationship between miR-125b-5p and KIAA1522. ***PK) KIAA1522 mRNA expression in Hep3b cells after mimic or inhibitor transfection was analyzed by RT-qPCR analysis. ***P###P

Figure 4. MiR-125b-5p directly targets KIAA1522. (A) The differentially expressed mRNAs in HCC were predicted by TCGA database. (B) The intersection of mRNAs combined with miR-125b-5p was shown by Venn diagram. (C) Expression of KIAA1522 and PODXL was increased in HCC tissues compared with normal tissues. (D) Impact of expression of KIAA1522 and PODXL on overall survival in HCC patients in TCGA cohort. (E) miR-125b-5p expression was negatively correlated with KIAA1522 expression. (F) KIAA1522 expression was increased in HCC tissues compared with normal tissues. (G) KIAA1522 expression in HCC tissues and adjacent tissues was detected by immunohistochemistry. (H) KIAA1522 mRNA expression in HCC cells and HHL-5 cells was analyzed by RT-qPCR analysis. ***P<0.001 vs. HHL-5 group. (I) The binding site between miR-125b-5p and KIAA1522 was predicted by ENCORI. (J) Luciferase reporter assay for the confirmation of direct binding relationship between miR-125b-5p and KIAA1522. ***P<0.001 vs. mimic-NC+KIAA1522 group. (K) KIAA1522 mRNA expression in Hep3b cells after mimic or inhibitor transfection was analyzed by RT-qPCR analysis. ***P<0.001 vs. mimic-NC group. ###P<0.001 vs. inhibitor-NC group.