Research Paper Volume 13, Issue 2 pp 2626—2639

LncRNA CYTOR affects the proliferation, cell cycle and apoptosis of hepatocellular carcinoma cells by regulating the miR-125b-5p/KIAA1522 axis

CYTOR affects proliferation, cell cycle and apoptosis of HCC cells by miR-125b-5p. (A) The proliferation of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by CCK-8 assay. *P#P###PB) The expression of proliferation-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. *P#P##PC) The cell cycle of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was analyzed by flow cytometry analysis. *P###PD) The apoptosis of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was shown by TUNEL assay. (E) The expression of cell cycle-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. ***P#P##P###PF, G) The expression of apoptosis-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. ***P##P###P

Figure 6. CYTOR affects proliferation, cell cycle and apoptosis of HCC cells by miR-125b-5p. (A) The proliferation of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by CCK-8 assay. *P<0.05 and ***P<0.001 vs. shRNA-NC+inhibitor-NC group. #P<0.05 and ###P<0.001 vs. shRNA-NC+miR-125b-5p inhibitor group. (B) The expression of proliferation-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. *P<0.05 and ***P<0.001 vs. shRNA-NC+inhibitor-NC group. #P<0.05 and ##P<0.01 vs. shRNA-NC+miR-125b-5p inhibitor group. (C) The cell cycle of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was analyzed by flow cytometry analysis. *P<0.05 and **P<0.01 vs. shRNA-NC+inhibitor-NC group. ###P<0.001 vs. shRNA-NC+miR-125b-5p inhibitor group. (D) The apoptosis of Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was shown by TUNEL assay. (E) The expression of cell cycle-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. ***P<0.001 vs. shRNA-NC+inhibitor-NC group. #P<0.05, ##P<0.01 and ###P<0.001 vs. shRNA-NC+miR-125b-5p inhibitor group. (F, G) The expression of apoptosis-related proteins in Hep3b cells after transfection of shRNA-CYTOR-1 and miR-125b-5p inhibitor was detected by Western blot analysis. ***P<0.001 vs. shRNA-NC+inhibitor-NC group. ##P<0.01 and ###P<0.001 vs. shRNA-NC+miR-125b-5p inhibitor group.