Research Paper Volume 13, Issue 2 pp 2626—2639

LncRNA CYTOR affects the proliferation, cell cycle and apoptosis of hepatocellular carcinoma cells by regulating the miR-125b-5p/KIAA1522 axis

MiR-125b-5p/KIAA1522 axis affects proliferation, cell cycle and apoptosis of HCC cells. (A) The proliferation of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by CCK-8 assay. **P#PB) The expression of proliferation-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P##P###PC) The cell cycle of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was analyzed by flow cytometry analysis. ***P###PD) The apoptosis of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was shown by TUNEL assay. (E) The expression of cell cycle-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P###PF, G) The expression of apoptosis-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P###P

Figure 7. MiR-125b-5p/KIAA1522 axis affects proliferation, cell cycle and apoptosis of HCC cells. (A) The proliferation of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by CCK-8 assay. **P<0.01 vs. mimic-NC+Oe-NC group. #P<0.05 vs. miR-125b-5p mimic+Oe-NC group. ΔP<0.05 vs. mimic-NC+Oe-KIAA1522 group. (B) The expression of proliferation-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P<0.05, **P<0.01 and ***P<0.001vs. mimic-NC+Oe-NC group. ##P<0.01 and ###P<0.001 vs. miR-125b-5p mimic+Oe-NC group. ΔP<0.05 vs. mimic-NC+Oe-KIAA1522 group. (C) The cell cycle of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was analyzed by flow cytometry analysis. ***P<0.001vs. mimic-NC+Oe-NC group. ###P<0.001 vs. miR-125b-5p mimic+Oe-NC group. ΔΔP<0.01 and ΔΔΔP<0.001 vs. mimic-NC+Oe-KIAA1522 group. (D) The apoptosis of Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was shown by TUNEL assay. (E) The expression of cell cycle-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P<0.05, **P<0.01 and ***P<0.001vs. mimic-NC+Oe-NC group. ###P<0.001 vs. miR-125b-5p mimic+Oe-NC group. ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. mimic-NC+Oe-KIAA1522 group. (F, G) The expression of apoptosis-related proteins in Hep3b cells after transfection of miR-125b-5p mimic and Oe-KIAA1522 was detected by Western blot analysis. *P<0.05, **P<0.01 and ***P<0.001vs. mimic-NC+Oe-NC group. ###P<0.001 vs. miR-125b-5p mimic+Oe-NC group. ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. mimic-NC+Oe-KIAA1522 group.