Figure 4. Fucoxanthin suppresses LPS-induced activation of NF-κB signaling in RAW 264.7 cells. (A) RAW264.7 cells were pretreated with the indicated concentrations of Fucoxanthin for 1 h before being stimulated with LPS for another 24 h. p-NF-κB was determined by immunofluorescence staining. DAPI-stained nuclei are indicated by blue fluorescence. NF-κB was indicated by red fluorescence, scale bar=200 μm. (B) The expression level of p-NF-κB p65 and NF-κB p65 was determined by immunoblotting. *P < 0.05, **P < 0.01 compared with the LPS alone group.