Figure 4. α-MSH-treatment induced ENST00000606533 and circ_0091223 expression but inhibits miR-1291 expression. (A–C) ENST00000606533, ENST00000532071, circ_0091223, circ_0031728, miR-1291, and miR-4530 levels were detected by qRT-PCR in MCs after 150 nM α-MSH treatment. (D) Luciferase reporter assay was used to detect the binding of miR-1291 to circ_0091223 and ENST00000606533. (E, F) Schematic model for wild type (WT) or mutant (mut) transcripts of ENST00000606533 or circ_0091223 luciferase reporters. (G) The binding site of miR-1291 with TYR predicted by TargetScan software.