Figure 10. Effects of melatonin on spindle formation and chromosome alignment in OBZ-exposed mice in vivo. (A) Representative images of spindle morphologies and chromosome alignment in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Green, α-tubulin; blue, DNA. (B) The rate of abnormal spindles observed in each treatment group. (C) Fluorescence intensity analysis of α-tubulin in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Values indicated by different letters are significantly different (P < 0.05). The experiments were repeated three times, with n = 15-20 per group. Control, untreated control group; OBZ, oxybenzone-exposed group; OBZ+MT, “oxybenzone + melatonin” treatment group.