Figure 4. Effects of melatonin on intracellular reactive oxygen species (ROS) levels, glutathione (GSH) levels, and early apoptosis in oxybenzone-exposed oocytes. (A) Representative images of ROS and GSH levels in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Scale bar, 100 μm. (B) Intracellular ROS and (C) GSH levels in mouse oocytes from each treatment group. (D) Representative images of normal oocytes and early apoptotic oocytes. Scale bar, 20 μm. (E) Proportion of early apoptotic oocytes in each treatment group. Values indicated by different letters are significantly different (P < 0.05). The experiments were repeated three times, with n = 15-20 per group. Control, untreated control group; OBZ, oxybenzone-exposed group; OBZ+MT, “oxybenzone + melatonin” treatment group.