Figure 6. Effects of melatonin on spindle formation and chromosome alignment in OBZ-exposed oocytes. (A) Representative images of spindle morphologies and chromosome alignment in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Green, α-tubulin; blue, DNA. (B) The rate of abnormal spindles in each treatment group. (C) Fluorescence intensity analysis of α-tubulin in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Values indicated by different letters are significantly different (P < 0.05). The experiments were repeated five times, with n = 10-15 per group. Control, untreated control group; OBZ, oxybenzone-exposed group; OBZ+MT, “oxybenzone + melatonin” treatment group.