Research Paper Volume 13, Issue 2 pp 2727—2749

Melatonin protects against oxybenzone-induced deterioration of mouse oocytes during maturation

Effects of melatonin on the H3K4me3 levels and expression of the Kdm5 family of genes in OBZ-exposed mice in vivo. (A) Immunofluorescence staining for H3K4me3 in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Green, H3K4me3; blue, DNA. Scale bar, 10 μm. (B) Average fluorescence intensity for H3K4me3 in mouse oocytes from the different groups. (C) Expression analysis of genes involved in histone H3K4me3 demethylation (Kdm5a, Kdm5b, and Kdm5c) from different groups. Values indicated by different letters are significantly different (P

Figure 8. Effects of melatonin on the H3K4me3 levels and expression of the Kdm5 family of genes in OBZ-exposed mice in vivo. (A) Immunofluorescence staining for H3K4me3 in the control, OBZ-exposed, and melatonin+OBZ-treated oocytes. Green, H3K4me3; blue, DNA. Scale bar, 10 μm. (B) Average fluorescence intensity for H3K4me3 in mouse oocytes from the different groups. (C) Expression analysis of genes involved in histone H3K4me3 demethylation (Kdm5a, Kdm5b, and Kdm5c) from different groups. Values indicated by different letters are significantly different (P < 0.05). The experiments were repeated five times, with n = 10-15 per group. Control, untreated control group; OBZ, oxybenzone-exposed group; OBZ+MT, “oxybenzone + melatonin” treatment group.