Research Paper Volume 13, Issue 2 pp 2768—2779

PTPN2 negatively regulates macrophage inflammation in atherosclerosis

PTPN2 deficiency promotes the proliferation, migration and invasion of THP-1 cells and U937 cells. (A, B) EDU assay and colony formation assay were used to analyze the proliferation ability of THP-1 cells and U937 cells. (C) Apoptosis of THP-1 cells and U937 cells were evaluated by flow cytometry. (D) MTT assay were used to examine the viability of HUVEC cell after incubated with THP-1 cells or U937 cells. (E, F) Transwell migration and invasion assays were performed in THP-1 cells and U937 cells. The upper is THP-1 cells and the lower is U937 cells in Figure 3E. The left is THP-1 cells and the right is U937 cells in Figure 3F. Bar= 20μM. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P

Figure 3. PTPN2 deficiency promotes the proliferation, migration and invasion of THP-1 cells and U937 cells. (A, B) EDU assay and colony formation assay were used to analyze the proliferation ability of THP-1 cells and U937 cells. (C) Apoptosis of THP-1 cells and U937 cells were evaluated by flow cytometry. (D) MTT assay were used to examine the viability of HUVEC cell after incubated with THP-1 cells or U937 cells. (E, F) Transwell migration and invasion assays were performed in THP-1 cells and U937 cells. The upper is THP-1 cells and the lower is U937 cells in Figure 3E. The left is THP-1 cells and the right is U937 cells in Figure 3F. Bar= 20μM. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P < 0.05. **, P < 0.01.