Research Paper Volume 13, Issue 2 pp 2768—2779

PTPN2 negatively regulates macrophage inflammation in atherosclerosis

PTPN2 can be a potential atherosis treatment target. PBS control and PTPN2 antibody were injected into ApoE-/- mice by tail vain injected. (A) Immunohistochemistry of CD68 in aortic roots of ApoE-/- mice which treated with PBS or PTPN2 antibody were performed. (B) ELISA assay was used to analyze the production of IL-6, IL-12 and IL-1β in macrophages. (C) The mRNA levels of IL-6, IL-12 and IL-1β in macrophages were analyzed by qRT-PCR assay. (D) IB assay was used to detect the expression of indicated proteins in macrophages. (E, F) Transwell migration and invasion assays in macrophages were performed. Bar= 20μM. (G) MTT assay was used to analyze the viability of HUVEC cell which incubated with macrophages. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P

Figure 5. PTPN2 can be a potential atherosis treatment target. PBS control and PTPN2 antibody were injected into ApoE-/- mice by tail vain injected. (A) Immunohistochemistry of CD68 in aortic roots of ApoE-/- mice which treated with PBS or PTPN2 antibody were performed. (B) ELISA assay was used to analyze the production of IL-6, IL-12 and IL-1β in macrophages. (C) The mRNA levels of IL-6, IL-12 and IL-1β in macrophages were analyzed by qRT-PCR assay. (D) IB assay was used to detect the expression of indicated proteins in macrophages. (E, F) Transwell migration and invasion assays in macrophages were performed. Bar= 20μM. (G) MTT assay was used to analyze the viability of HUVEC cell which incubated with macrophages. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not statistically significant; *, P < 0.05. **, P < 0.01.