Research Paper Volume 13, Issue 2 pp 2803—2821

SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma


Figure 4. Functional analysis of SPC25 overexpression in HCC cells. (A) The protein levels of SPC25 were detected by western blot analysis in SPC25 or vector-transfected cells. β-actin was used as an endogenous control. (B) CCK-8 assays, (C) colony formation, and (D) non-adherent colony formation assays demonstrated that overexpression of SPC25 promoted proliferation of Huh7 and PLC8024. (E) Images of xenograft tumors derived from SPC25-transfected cells and their vectors in nude mice. Tumor sizes were compared in the right chart. (*P < 0.05, **P < 0.01, ***P < 0.001).