Figure 4. Functional analysis of SPC25 overexpression in HCC cells. (A) The protein levels of SPC25 were detected by western blot analysis in SPC25 or vector-transfected cells. β-actin was used as an endogenous control. (B) CCK-8 assays, (C) colony formation, and (D) non-adherent colony formation assays demonstrated that overexpression of SPC25 promoted proliferation of Huh7 and PLC8024. (E) Images of xenograft tumors derived from SPC25-transfected cells and their vectors in nude mice. Tumor sizes were compared in the right chart. (*P < 0.05, **P < 0.01, ***P < 0.001).