Figure 6. miRNAs that down-regulated SPC25 expression in HCC. (A) Two miRNA prediction datasets, starBase, and Targetscan were used to predict miRNAs that may bind to SPC25 mRNA. (B) miRNAs negatively correlated with SPC25 mRNA expression (data from Linkomics). (C) Correlation between SPC25 mRNA and miR-451 expression. (D) Dual-luciferase reporter assay for confirmation of the targeting relationship between miR-451a and SPC25; *P < 0.05; statistical data were presented as mean ± standard deviation; data between two groups were analyzed by paired t-test; one-way analysis of variance was used for multi-group comparisons; the experiment was repeated three times independently, NC negative control.