Research Paper Volume 13, Issue 3 pp 4428—4451

Tsukushi is a novel prognostic biomarker and correlates with tumor-infiltrating B cells in non-small cell lung cancer

Correlations between TSKU expression and infiltrating B cell and DC levels in LUAD and LUSC. Gene markers include CD19, CD20, CD21, and CD40L for B cells; HLA-DPB1, HLA-DQB1, and HLA-DRA for dendritic cells. (A, B) Scatterplots of correlations between TSKU expression and gene markers of B cells (A) and DCs (B) in LUAD (N = 515). (C, D) Scatterplots of correlations between TSKU expression and gene markers of B cells (C) and DCs (D) in LUSC (N = 501). (E) Patients with low infiltrating levels of B cell and dendritic cell showed a poor survival in LUAD (B cell, N =496) (dendritic cell, N =501). (F) Patients with high infiltrating levels of CD4+ T cell showed a poor survival in LUSC (CD4+ T cell, N =492). (G) The survival of patients with high or low TSKU expression and high or low infiltrating B cell levels in NSCLC (N=480). (H) The survival of patients with high or low TSKU expression and high or low infiltrating DC levels in NSCLC (N=480). (The marked blue means high TSKU expression and low B cells (or DCs) infiltration (N=120); marked green means low TSKU expression and high B cells (or DCs) infiltration (N=120); marked red means low TSKU expression and low B cells (or DCs) infiltration (N=120); marked purple means high TSKU expression and high B cells (or DCs) infiltration (N=120)).

Figure 4. Correlations between TSKU expression and infiltrating B cell and DC levels in LUAD and LUSC. Gene markers include CD19, CD20, CD21, and CD40L for B cells; HLA-DPB1, HLA-DQB1, and HLA-DRA for dendritic cells. (A, B) Scatterplots of correlations between TSKU expression and gene markers of B cells (A) and DCs (B) in LUAD (N = 515). (C, D) Scatterplots of correlations between TSKU expression and gene markers of B cells (C) and DCs (D) in LUSC (N = 501). (E) Patients with low infiltrating levels of B cell and dendritic cell showed a poor survival in LUAD (B cell, N =496) (dendritic cell, N =501). (F) Patients with high infiltrating levels of CD4+ T cell showed a poor survival in LUSC (CD4+ T cell, N =492). (G) The survival of patients with high or low TSKU expression and high or low infiltrating B cell levels in NSCLC (N=480). (H) The survival of patients with high or low TSKU expression and high or low infiltrating DC levels in NSCLC (N=480). (The marked blue means high TSKU expression and low B cells (or DCs) infiltration (N=120); marked green means low TSKU expression and high B cells (or DCs) infiltration (N=120); marked red means low TSKU expression and low B cells (or DCs) infiltration (N=120); marked purple means high TSKU expression and high B cells (or DCs) infiltration (N=120)).