Figure 6. Changes of F-actin and p-MLC in each group in in vitro experimental groups. (A) Anti-VAP-1 antibody treatment induces a loss of stress fibers and p-MLC in isolated LSECs after coculture for 3 h, however anti-integrin α4 antibody treatment produces the opposite effect. F-actin (red) staining with Alexa Fluor 555 and p-MLC (green) staining with Alexa Fluor 488 were examined by confocal immunofluorescence microscopy. Nuclei (blue) were counterstained with DAPI. The scale bar denotes 20 μm. (B) Quantification of F-actin and p-MLC expression of each group in (A). ***p < 0.001.