Research Paper Volume 13, Issue 2 pp 2982—3009

Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome

Activation of fibrosis-related signaling pathways can be prohibited by β-catenin inhibitor IGC001 (5 mg/kg/d) in the kidneys at 12 weeks after MI. (A) Representative photomicrographs of renal fibrosis detected by Masson’s trichrome stain in sham mice or CRS mice treatment with IGC001 or DMSO. Semi-quantitative assessment of glomerular fibrosis (B) and interstitial fibrosis (C). **P ***P D) Western blot of collagen I, vimentin and TGF- β. (E) Western blot of p-GSK-3β and active-β-catenin. *P **P ***P

Figure 5. Activation of fibrosis-related signaling pathways can be prohibited by β-catenin inhibitor IGC001 (5 mg/kg/d) in the kidneys at 12 weeks after MI. (A) Representative photomicrographs of renal fibrosis detected by Masson’s trichrome stain in sham mice or CRS mice treatment with IGC001 or DMSO. Semi-quantitative assessment of glomerular fibrosis (B) and interstitial fibrosis (C). **P < 0.01, ***P < 0.001. NS, no statistical significance, n = 5 per group. (D) Western blot of collagen I, vimentin and TGF- β. (E) Western blot of p-GSK-3β and active-β-catenin. *P < 0.05. **P < 0.01, ***P < 0.001. NS, no statistical significance, n = 3 per group. Data are means ± SE.