Figure 6. Recombinant FGF23 promotes activation of fibrosis-related signaling pathways in NRK-49F cultured kidney fibroblasts. (A) Expression of Klotho mRNA was not detected. Western blotting showed that FGFR4 protein expression was upregulated by treatment with recombinant FGF23 (100 ng/mL) or angiotensin II (Ang II, 1 μM). (B) Western blot of collagen I. (C) Western blot of vimentin. (D) Western blot of TGF-β. (E) Western blot of p-GSK-3β. (F) Western blot of active-β-catenin. Con, control; FGF23, fibroblast growth factor 23; Ang II, angiotensin II. *P < 0.05, **P < 0.01, n = 3-6 per group. Data are means ± SE.