Research Paper Volume 13, Issue 2 pp 2982—3009

Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome

Profibrotic effect of FGF23 can be inhibited by treatment with FGFR4 inhibitor BLU9931 or β-catenin inhibitor IGC001. (A) Western blot of p-GSK-3β. (B) Western blot of active-β-catenin. (C) Western blot of TGF-β. (D) Western blot of collagen I. These representative blots were performed in the same gel as (A), so that both panels shared the same loading controls. (E) Western blot of vimentin. BLU9931, 100 nM; IGC001, 5 μM. *P **P

Figure 7. Profibrotic effect of FGF23 can be inhibited by treatment with FGFR4 inhibitor BLU9931 or β-catenin inhibitor IGC001. (A) Western blot of p-GSK-3β. (B) Western blot of active-β-catenin. (C) Western blot of TGF-β. (D) Western blot of collagen I. These representative blots were performed in the same gel as (A), so that both panels shared the same loading controls. (E) Western blot of vimentin. BLU9931, 100 nM; IGC001, 5 μM. *P < 0.05, **P < 0.01, n = 3-5 per group. Data are means ± SE.