Research Paper Volume 13, Issue 2 pp 3045—3059

Transplantation of bone marrow-derived mesenchymal stem cells with silencing of microRNA-138 relieves pelvic organ prolapse through the FBLN5/IL-1β/elastin pathway

miR-138 inhibition improves results of urodynamic tests in PFD rats after BMSC transplant. (A) miR-138 expression in BMSCs treated with antagomir-NC or miR-138 antagomir evaluated by RT-qPCR; (B) basal bladder pressure level of rats in each group; (C) bladder void volume of rats in each group; (D) bladder void pressure of rats in each group; (E) peak bladder pressure of rats in each group; (F) LPP of rats in each group; (G) the protein expression of IL-1β, elastin, and FBNL5 in rats evaluated by western blot analysis; (H) the content of IL-1β and elastin in peripheral blood of rats examined by ELISA. *p vs. BMSCs treated with antagomir-NC or rats injected with saline (control rats); #p vs. PFD rats injected with BMSCsantagomir-NC; statistical data were measurement data, and described as mean ± standard deviation. The unpaired t test was conducted for comparison between two groups. The one-way ANOVA was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.

Figure 4. miR-138 inhibition improves results of urodynamic tests in PFD rats after BMSC transplant. (A) miR-138 expression in BMSCs treated with antagomir-NC or miR-138 antagomir evaluated by RT-qPCR; (B) basal bladder pressure level of rats in each group; (C) bladder void volume of rats in each group; (D) bladder void pressure of rats in each group; (E) peak bladder pressure of rats in each group; (F) LPP of rats in each group; (G) the protein expression of IL-1β, elastin, and FBNL5 in rats evaluated by western blot analysis; (H) the content of IL-1β and elastin in peripheral blood of rats examined by ELISA. *p < 0.05 vs. BMSCs treated with antagomir-NC or rats injected with saline (control rats); #p < 0.05 vs. PFD rats injected with BMSCsantagomir-NC; statistical data were measurement data, and described as mean ± standard deviation. The unpaired t test was conducted for comparison between two groups. The one-way ANOVA was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated 3 times independently.