Research Paper Volume 13, Issue 2 pp 3101—3111

Sleep deprivation aggravates brain injury after experimental subarachnoid hemorrhage via TLR4-MyD88 pathway

Involvement of TLR4-MyD88 pathway in sleep deprivation-induced aggravation of brain injury after SAH. (A) Brain water content assay shows that aggravation of brain edema induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. (B) TUNEL staining shows that the increase in apoptosis induced by sleep deprivation after SAH was inhibited by TAK-242 and ST2825. (C–E) Western blot (C) and quantification (D, E) show that the increased expression of C-caspase-3 (D) and Iba-1 (E) induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. (F–I) ELISA shows that the increased levels of IL-1β (F) and TNF-α (G) and decreased the levels of IL-10 (H) and TGF-β1 (I) induced by sleep deprivation after SAH were reversed by TAK-242 and ST2825. (J) Neurological score assay shows that aggravation of neurological impairment induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. The data was represented as means ± SEM. #p *p &p

Figure 5. Involvement of TLR4-MyD88 pathway in sleep deprivation-induced aggravation of brain injury after SAH. (A) Brain water content assay shows that aggravation of brain edema induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. (B) TUNEL staining shows that the increase in apoptosis induced by sleep deprivation after SAH was inhibited by TAK-242 and ST2825. (CE) Western blot (C) and quantification (D, E) show that the increased expression of C-caspase-3 (D) and Iba-1 (E) induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. (FI) ELISA shows that the increased levels of IL-1β (F) and TNF-α (G) and decreased the levels of IL-10 (H) and TGF-β1 (I) induced by sleep deprivation after SAH were reversed by TAK-242 and ST2825. (J) Neurological score assay shows that aggravation of neurological impairment induced by sleep deprivation after SAH was prevented by TAK-242 and ST2825. The data was represented as means ± SEM. #p < 0.05 vs. Sham group, *p < 0.05 vs. SAH group and &p < 0.05 vs. SAH+SD group.