Research Paper Volume 13, Issue 5 pp 6712—6723

CPI-1189 protects neuronal cells from oxygen glucose deprivation/re-oxygenation-induced oxidative injury and cell death

CPI-1189 protects neuronal cells from OGDR-induced cell death. SH-SY5Y neuronal cells (A–C) or primary murine cortical neurons (D–F) were pretreated for 1h with CPI-1189 (at applied concentrations) and subjected to OGDR procedure, cells were cultured for applied time periods, cell viability, cell death and p38 activation were tested by CCK-8 (A–D), Trypan blue staining (B–E) and Western blotting (C–F) assays, respectively. “Mock” stands for neuronal cells placed in norm-oxygenated regular medium containing glucose (same for all Figures). Quantified values were mean ± standard deviation (SD, n=5). * P vs. “Mock” cells. #P vs. cells with OGDR stimulation but “DMSO (0.1%)” pretreatment. Experiments were repeated three times, with similar results obtained.

Figure 1. CPI-1189 protects neuronal cells from OGDR-induced cell death. SH-SY5Y neuronal cells (AC) or primary murine cortical neurons (DF) were pretreated for 1h with CPI-1189 (at applied concentrations) and subjected to OGDR procedure, cells were cultured for applied time periods, cell viability, cell death and p38 activation were tested by CCK-8 (AD), Trypan blue staining (BE) and Western blotting (CF) assays, respectively. “Mock” stands for neuronal cells placed in norm-oxygenated regular medium containing glucose (same for all Figures). Quantified values were mean ± standard deviation (SD, n=5). * P < 0.05 vs. “Mock” cells. #P < 0.05 vs. cells with OGDR stimulation but “DMSO (0.1%)” pretreatment. Experiments were repeated three times, with similar results obtained.