Research Paper Volume 13, Issue 2 pp 1692—1717

A pro-diabetogenic mtDNA polymorphism in the mitochondrial-derived peptide, MOTS-c

K14Q MOTS-c is less effective than WT MOTS-c, on reducing body weight, fat mass, and glucose tolerance in CD1 male mice exposed to high-fat diet. (A–D) Male CD-1 mice (14 weeks old) fed a high-fat diet (HFD, 60% by calories) (n = 8-12) treated with WT and K14Q MOTS-c (7.5 mg/kg; IP; BID) for 21 days. (A) body weight, (B) fat mass, (C) lean mass, (D) food intake. (E, F) Eight-weeks old male CD-1 mice fed a HFD diet (n = 8-10) treated with WT and K14Q MOTS-c (0.5 mg/kg; IP; daily) for 21 days, at which point they were assessed with a glucose tolerance test (GTT). Shown are: (E) Blood glucose and (F) glucose AUC, during the GTT. (A–D) *** p p p E, F) *p p

Figure 2. K14Q MOTS-c is less effective than WT MOTS-c, on reducing body weight, fat mass, and glucose tolerance in CD1 male mice exposed to high-fat diet. (AD) Male CD-1 mice (14 weeks old) fed a high-fat diet (HFD, 60% by calories) (n = 8-12) treated with WT and K14Q MOTS-c (7.5 mg/kg; IP; BID) for 21 days. (A) body weight, (B) fat mass, (C) lean mass, (D) food intake. (E, F) Eight-weeks old male CD-1 mice fed a HFD diet (n = 8-10) treated with WT and K14Q MOTS-c (0.5 mg/kg; IP; daily) for 21 days, at which point they were assessed with a glucose tolerance test (GTT). Shown are: (E) Blood glucose and (F) glucose AUC, during the GTT. (AD) *** p < 0.001, ** p < 0.01, *p < 0.05. (E, F) *p < 0.01 Water vs. WT MOTS-c. †p < 0.01, K14Q MOTS- vs. WT MOTS-c.