Research Paper Volume 13, Issue 5 pp 6724—6739

MiR-506 exerts antineoplastic effects on osteosarcoma cells via inhibition of the Skp2 oncoprotein

Skp2 downregulation enhances miR-506-mediated viability inhibition and apoptosis induction. (A) Viability was examined by MTT assay in cells after miR-506 overexpression and Skp2 downregulation. *p #PB) Apoptosis was examined by flow cytometry in osteosarcoma cells after miR-506 mimic transfection and Skp2 downregulation. (C) Quantification of apoptosis results.

Figure 7. Skp2 downregulation enhances miR-506-mediated viability inhibition and apoptosis induction. (A) Viability was examined by MTT assay in cells after miR-506 overexpression and Skp2 downregulation. *p < 0.05 vs control, #P<0.05 vs miR-506 mimics or Skp2 siRNA transfection. (B) Apoptosis was examined by flow cytometry in osteosarcoma cells after miR-506 mimic transfection and Skp2 downregulation. (C) Quantification of apoptosis results.