Research Paper Volume 13, Issue 6 pp 8497—8509

Linc00641 promotes the progression of gastric carcinoma by modulating the miR-429/Notch-1 axis

Effects of linc00641 overexpression on migration and invasion. (A) The potential interaction between miR-429 and Notch-1 is shown. Dual luciferase reporter assays were used to validate the targeting of miR-429 to wild-type Notch-1 in BGC-823 cells. (B) Western blot analysis was applied to examine Notch-1 expression in gastric cancer cells after linc00641 modulation. (C) The cell viability was tested by MTT assays after linc00641 siRNA and Notch-1 cDNA cotransfection. *P # P D) Cell viability was detected by MTT assays in gastric cancer cells with linc00641 overexpression and Notch-1 downregulation. *P # P

Figure 6. Effects of linc00641 overexpression on migration and invasion. (A) The potential interaction between miR-429 and Notch-1 is shown. Dual luciferase reporter assays were used to validate the targeting of miR-429 to wild-type Notch-1 in BGC-823 cells. (B) Western blot analysis was applied to examine Notch-1 expression in gastric cancer cells after linc00641 modulation. (C) The cell viability was tested by MTT assays after linc00641 siRNA and Notch-1 cDNA cotransfection. *P < 0.05 compared with control, # P < 0.05 compared with linc00641 siRNA or Notch-1 cDNA. (D) Cell viability was detected by MTT assays in gastric cancer cells with linc00641 overexpression and Notch-1 downregulation. *P < 0.05 compared with control shRNA, # P < 0.05 compared with Notch-1 siRNA or linc00641.