Research Paper Volume 13, Issue 4 pp 4946—4961

Fibroblasts from different body parts exhibit distinct phenotypes in adult progeria Werner syndrome

Osteogenesis was maintained in both groups. (A) Representative images of Alizarin red staining two weeks after induction of osteogenesis in the trunk and foot fibroblasts of WS1. Bar = 300 μm. (B) Quantification of relative stained area with Alizarin red. Data are means ± SEM of two patients with four microscopic views. For statistical analysis, student t-test was performed (ns, not significant). (C) Relative gene expression analyzed by qRT-PCR. Data are means ± SEM of two patients with three technical replicates. For statistical analysis, student t-test was performed (ns, not significant; *p

Figure 5. Osteogenesis was maintained in both groups. (A) Representative images of Alizarin red staining two weeks after induction of osteogenesis in the trunk and foot fibroblasts of WS1. Bar = 300 μm. (B) Quantification of relative stained area with Alizarin red. Data are means ± SEM of two patients with four microscopic views. For statistical analysis, student t-test was performed (ns, not significant). (C) Relative gene expression analyzed by qRT-PCR. Data are means ± SEM of two patients with three technical replicates. For statistical analysis, student t-test was performed (ns, not significant; *p<0.05; **p<0.01).