Figure 1. Characterization of hsa_circ_0053063 in BC cells. (A) Hsa_circ_0053063 is composed of exon 5-7 of gene HADHA. (B) qRT-PCR was used to test the expression of hsa_circ_0053063 in MDA-MB-231 and MDA-MB-468 cells with random hexamer or oligo (dT)18 primers. (C–D) qRT-PCR analysis revealed the expression of hsa_circ_0053063, linear HADHA, and β-actin in MDA-MB-231 and MDA-MB-468 cells treated with or without RNase R. (E–F) Expression of PDCD4 mRNA level and hsa_circ_0053063 in MDA-MB-231 and MDA-MB-468 cells treated with actinomycin D. (G–H) Expression levels of GAPDH, U6, and hsa_circ_0053063 in the cell cytoplasmic and nuclear were determined by qRT-PCR in MDA-MB-231 and MDA-MB-468 cells. (I) RNA FISH for hsa_circ_0053063, and nuclei was stained with DAPI. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).