Figure 4. Hsa_circ_0053063 inhibited the expression of miR-330-3p. (A–B) Databases were employed to predict potential miRNA target of hsa_circ_0053063 and Venn diagram showed the target miRNAs from intersections (miR-330-3p, miR-346, miR-431-5p, miR-874-3p, miR-1182). (C–D) qRT-PCR was used to verify which miRNAs can be regulated by hsa_circ_053063 in MDA-MB-231 and MDA-MB-468 cell lines. (E) Predicted binding sites of hsa_circ_0053063 for miR-330-3p. (F) Dual-luciferase reporter assay evaluating the interaction between wild-type hsa_circ_0053063 + miR-330-3p reporter compared with mutant hsa_circ_0053063+ miR-330-3p reporter. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).