Research Paper Volume 13, Issue 6 pp 9011—9027

Functional genomics study of protein inhibitor of activated STAT1 in mouse hippocampal neuronal cells revealed by RNA sequencing

Validation of target gene expression by qRT-PCR. (A) For upregulated DEGs, 11 genes were selected for the following qRT-PCR validation. (B) For downregulated DEGs, 18 genes were selected for the following qRT-PCR validation. (C) The PIAS1 regulation of cell cycle inhibitors, including p15, p18, p21, p27, p53 and p57, was tested by qRT-PCR. Among them, three inhibitors, p53, p18 and p21, were significantly downregulated under the overexpression of Pias1. *p **p

Figure 4. Validation of target gene expression by qRT-PCR. (A) For upregulated DEGs, 11 genes were selected for the following qRT-PCR validation. (B) For downregulated DEGs, 18 genes were selected for the following qRT-PCR validation. (C) The PIAS1 regulation of cell cycle inhibitors, including p15, p18, p21, p27, p53 and p57, was tested by qRT-PCR. Among them, three inhibitors, p53, p18 and p21, were significantly downregulated under the overexpression of Pias1. *p < 0.05, **p < 0.01.