Research Paper Advance Articles

Human adipose-derived stem cells promote seawater-immersed wound healing via proangiogenic effects

Effect of hADSCs on the proliferation and migration of HUVEC in vitro. (A) CCK8 assay results showed that the proliferation of HUVEC was significantly inhibited following exposure to 10% seawater concentration. (B) transwell coculture system schematic. (C) the effects of seawater and hADSC on the protein and mRNA expression of VEGFR in HUVEC. (D–E) the effects of seawater and hADSC on HUVEC proliferation. Proliferating HUVEC were stained with green fluorescence, and all nuclei were stained with blue fluorescence. Scale bars indicate 100 μm. (F–G) the effects of seawater and hADSC on HUVEC migration. *P **P ***P

Figure 6. Effect of hADSCs on the proliferation and migration of HUVEC in vitro. (A) CCK8 assay results showed that the proliferation of HUVEC was significantly inhibited following exposure to 10% seawater concentration. (B) transwell coculture system schematic. (C) the effects of seawater and hADSC on the protein and mRNA expression of VEGFR in HUVEC. (D–E) the effects of seawater and hADSC on HUVEC proliferation. Proliferating HUVEC were stained with green fluorescence, and all nuclei were stained with blue fluorescence. Scale bars indicate 100 μm. (F–G) the effects of seawater and hADSC on HUVEC migration. *P < 0:05, **P < 0:01 and ***P < 0:001.