Research Paper Volume 13, Issue 7 pp 10603—10618

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma

DCAF15 inhibits proliferation, migration, and invasion of HCC cells. (A) DCAF15 and ZEB1 was knocked down in SMMC-7721 cells by expressing siRNAs. Immunoblotting analyses were performed with the indicated antibodies. (B) CCK-8 assay was performed for cell proliferation of control, ZEB1-knockdown, DCAF15-knockdown and ZEB1-& DCAF15-knockdown SMMC-7721 cells. (C) The cells of (B) were plated and cultured on 6-well plates. Colony numbers were quantified. (D) The migration and invasion ability of the cells of (B) in were examined using the Transwell migration and invasion assay respectively. Cell numbers were quantified. Data are presented as mean ± SD (n = 3). **P ***P E) The cells of (B) were plated and cultured on 6-well plates. The cell layer was scratched with a 10 μl pipette tip. For each sample, at least three scratched fields were photographed immediately (0 h) or 24 h after scratching. Photographs of representative images were taken at ×100 magnification.

Figure 5. DCAF15 inhibits proliferation, migration, and invasion of HCC cells. (A) DCAF15 and ZEB1 was knocked down in SMMC-7721 cells by expressing siRNAs. Immunoblotting analyses were performed with the indicated antibodies. (B) CCK-8 assay was performed for cell proliferation of control, ZEB1-knockdown, DCAF15-knockdown and ZEB1-& DCAF15-knockdown SMMC-7721 cells. (C) The cells of (B) were plated and cultured on 6-well plates. Colony numbers were quantified. (D) The migration and invasion ability of the cells of (B) in were examined using the Transwell migration and invasion assay respectively. Cell numbers were quantified. Data are presented as mean ± SD (n = 3). **P < 0.01, ***P < 0.001. Scale bars, 50 μm. (E) The cells of (B) were plated and cultured on 6-well plates. The cell layer was scratched with a 10 μl pipette tip. For each sample, at least three scratched fields were photographed immediately (0 h) or 24 h after scratching. Photographs of representative images were taken at ×100 magnification.