Figure 3. Verification of the interaction between candidate miRNAs and the 3′-UTR of the S100B gene, which contains the rs9722 loci, using the luciferase assay. (A) The candidate miRNAs that can bind to the rs9722 locus in the 3′-UTR of the S100B gene. The red letters show the rs9722 locus. (B) Plasmids containing different genotypes and artificial mutations. The black frame indicates the rs9722 locus. (C) Detection of miRNA levels in the 293T cells after transfection. (D) Fluorescence intensity after transfection of the miRNAs and the plasmids containing the different rs9722 alleles or mutations (*P < 0.05).