Figure 1. Antrodia salmonea (AS) inhibits the growth and induces intracellular ROS generation in human colon cancer cells. (A–C) SW620, HCT116, and HT-29 cells were treated with AS (0–300 μg/mL) for 24 h. Cell viability was determined using the MTT assay. (D, E) Cells were treated with 200 μg/mL AS for 0–120 min. 10 μM of DCFH2-DA was mixed in the culture medium 30 min before of each experiment and then intracellular ROS levels were measured and expressed in graph as a fold of the control. Each value is expressed as the mean ± SD (n = 3) and significant at *p < 0.05; **p < 0.01; and ***p < 0.001 when compared with control.