Research Paper Volume 13, Issue 12 pp 15964—15989
Antrodia salmonea induces apoptosis and enhances cytoprotective autophagy in colon cancer cells
Figure 10. Inhibitory effects of AS on AKT, mTOR, and NFκB signaling pathways in SW620 cells. (A) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of p(Ser473)-AKT and AKT. (B) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of p(Ser2448)-mTOR and mTOR. (C) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of nuclear and cytosolic NFκB (p65). (D) Cells were treated with AS (100, 150, and 200 μg/mL) for 24 h. Immunoblotting was performed to measure the levels of nuclear and cytosolic β-catenin. Relative changes in the intensities of protein bands were measured using commercially available quantitative software with the control representing as 1-fold.