Research Paper Volume 13, Issue 12 pp 15964—15989

Antrodia salmonea induces apoptosis and enhances cytoprotective autophagy in colon cancer cells


Figure 9.

ERK activation inhibited apoptosis through enhanced cytoprotective autophagy in AS-treated SW620 cells. (A) Cells were treated with AS (200 μg/mL) for 0–24 h and immunoblotting was performed to measure the levels of p(Thr202/Tyr204)-ERK1/2 and ERK1/2. Cells were pretreated with or without ERK inhibitor (PD98059, 10 μM) for 1 h, followed by 200 μg/mL AS treatment for 24 h. (B) The expressions of LC3-I/II and (C) caspase-3 were assessed by Western blotting. Relative changes in the expression of protein bands were measured by commercially available quantitative software with the control representing as 1-fold. (D) Cell viability was measured by the MTT assay. Earlier the cells were treated with or without ERK inhibitor (PD98059, 10 μM) for 1 h, and then followed by AS (100, 150, and 200 μg/mL) treatment for 24 h. Results are expressed as the mean ± SD of three independent assays. #p < 0.05 is significant when compared with AS-treated cells.