Figure 4. Overexpression of AK136714 protects the endothelial barrier and inhibits the inflammatory response of endothelial cells. (A) Detection of endothelial cell permeability. (B, C) Western blot and immunofluorescence were performed to detect endothelium tight junction biomarkers. (D) ELISA detection of inflammatory factor expression. (E) qPCR detection of inflammatory factor expression. (n=6, *P < 0.05).