Figure 5. RUSC1-AS1/miR-340-5p activated PI3K/AKT signaling pathway. (A) KEGG analysis of the target genes of miR-340-5p predicted by miRDB website tool. (B–C) Western blot analysis of proteins in PI3K/AKT pathway in U2OS/HOS cells or U2OS/HOS cells transfected with RUSC1-AS1 plasmid, miR-mimics or RUSC1-AS1+miR-mimics. (D–E) CCK-8 assay in U2OS/HOS cells transfected with RUSC1-AS1 plasmid with/without wortmannin treatment.