Research Paper Volume 13, Issue 13 pp 16938—16956

Macrophage phenotype and function are dependent upon the composition and biomechanics of the local cardiac tissue microenvironment

Differentially aged decellularized cECM promotes altered gene expression patterns in macrophage populations. (A) Fold change qRT-PCR results of pro- (Nos2, Tnfa) and anti-inflammatory (Tgfb, Arg) gene expression in RNA isolated from whole heart tissue lysates isolated from either young (1 month), moderately aged (8 months) or advanced age (18-21 month) C57/Bl6 mice (n=3-5). (B) Heatmap of qRT-PCR results for pro- and anti-inflammatory gene expression in macrophages isolated from young mice (8-16 week) cultured on tissue culture plastic coated with either young(Y, 8-16 week) or aged(A, 18-21 month) cECM with either no supplemental cytokine treatment (M0, YM0, AM0), Th1 cytokine treatment (M1, YM1, AM1), or Th2 cytokine treatment (M2, YM2, AM2) (n=4). Significant effects are noted below heatmap in boxplots with data reported as mean values with boxes representing 25%/75% of mean. Error bars represent standard deviation. Significance was assessed using a Kruskal-Wallis 1-way ANOVA with post-hoc pairwise comparisons. * p

Figure 5. Differentially aged decellularized cECM promotes altered gene expression patterns in macrophage populations. (A) Fold change qRT-PCR results of pro- (Nos2, Tnfa) and anti-inflammatory (Tgfb, Arg) gene expression in RNA isolated from whole heart tissue lysates isolated from either young (1 month), moderately aged (8 months) or advanced age (18-21 month) C57/Bl6 mice (n=3-5). (B) Heatmap of qRT-PCR results for pro- and anti-inflammatory gene expression in macrophages isolated from young mice (8-16 week) cultured on tissue culture plastic coated with either young(Y, 8-16 week) or aged(A, 18-21 month) cECM with either no supplemental cytokine treatment (M0, YM0, AM0), Th1 cytokine treatment (M1, YM1, AM1), or Th2 cytokine treatment (M2, YM2, AM2) (n=4). Significant effects are noted below heatmap in boxplots with data reported as mean values with boxes representing 25%/75% of mean. Error bars represent standard deviation. Significance was assessed using a Kruskal-Wallis 1-way ANOVA with post-hoc pairwise comparisons. * p<0.05.