Research Paper Volume 13, Issue 12 pp 15990—16008

Comprehensive analysis of the competing endogenous circRNA-lncRNA-miRNA-mRNA network and identification of a novel potential biomarker for hepatocellular carcinoma


Figure 7. Validation of DTYMK’s expression and function. (A) The expression levels of DTYMK in HCC and adjacent noncancer tissues were evaluated by Western blot (n=47). (B) Statistical analysis of relative DTYMK levels in HCC tissues compared to normal tissue controls (n= 47). (C, D) Transfection efficiency was verified after transfection of siDTYMK or negative control siRNA. (E) HCC cell viability was evaluated with CCK-8 assays. (F) EdU assay showed change of proliferative rate after transfection with siDTYMK. (G) The number of HCC cell colonies was reduced after transfection with siDTYMK. *** represents p < 0.001, ** represents P < 0.01, * represents P < 0.05.