Research Paper Advance Articles

Hypoxia induces chemoresistance of esophageal cancer cells to cisplatin through regulating the lncRNA-EMS/miR-758-3p/WTAP axis

Targeted knockdown of EMS or WTAP significantly enhanced the therapeutic effects of DDP in tumor eradication. Nude mice were inoculated with EMS-silenced or WTAP-silenced ECA-109 cells and treated with DDP. Tumor growth curves (A), tumor weight (B), cell proliferation (C), and apoptosis (D) of tumor tissues, as well as the RNA/transcript levels of EMS, miR-758-3p, and WTAP (E), the protein level of WTAP (F), and mRNA levels of mouse IL-10 (G) in tumor tissues are shown. For tumor volume comparisons (A), sh-NC+Vehicle, sh-EMS+Vehicle, or sh-WTAP+Vehicle group was compared with their corresponding DDP treatment group, respectively. n=5 mice in each group, and representative data are from at least 5 experiments with similar results. n=5 for each group.

Figure 7. Targeted knockdown of EMS or WTAP significantly enhanced the therapeutic effects of DDP in tumor eradication. Nude mice were inoculated with EMS-silenced or WTAP-silenced ECA-109 cells and treated with DDP. Tumor growth curves (A), tumor weight (B), cell proliferation (C), and apoptosis (D) of tumor tissues, as well as the RNA/transcript levels of EMS, miR-758-3p, and WTAP (E), the protein level of WTAP (F), and mRNA levels of mouse IL-10 (G) in tumor tissues are shown. For tumor volume comparisons (A), sh-NC+Vehicle, sh-EMS+Vehicle, or sh-WTAP+Vehicle group was compared with their corresponding DDP treatment group, respectively. n=5 mice in each group, and representative data are from at least 5 experiments with similar results. n=5 for each group.