Research Paper Volume 13, Issue 11 pp 15444—15458

Alterations in the RTK/Ras/PI3K/AKT pathway serve as potential biomarkers for immunotherapy outcome of diffuse gliomas


Figure 3. Correlations between RTK/Ras/PI3K/AKT signaling and immune-regulatory genes mRNA signatures. (A) The immune checkpoint genes were significantly upregulated in the activated RTK/Ras/PI3K/AKT signaling. (B) GSEA analysis showed a prominent enrichment of IFNγ pathways and PI3K-AKT-mTOR. (C) The single cell RNA seq showed a significant correlation between PI3K-AKT-mTOR and IFNγ pathway. The glioma cells with detectable CD274 (D) or PDCD1LG2 (E) showed an elevated activity of “PI3K_AKT_MTOR_SIGNALING”. (F) The PI3K_AKT_MTOR_SIGNALING pathway activity was significantly increased in tumor-associated macrophages, instead of T cells.