Figure 2. SIRT1 expression was decreased in BMDMs exposed to HDM. Dose–dependent expression of SIRT1 in bone marrow–derived macrophages (BMDMs) stimulated with HDM for 24 h and was analyzed using RT-PCR (A) and WB (C); Time–dependent expression of SIRT1 in BMDMs treated with HDM at 100 μg/ml and was analyzed using RT-PCR (B) and Western blot (D); BMDMs treated with HDM at 100 μg/ml for 24 h. Representative immunofluorescence images of SIRT1 (red) expression in BMDMs (E, Scale bar, 50 μm) and semiquantification of immunofluorescence images was done using Image Pro 6.1 software (F). Data are presented as Mean ± SEM of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001.