Figure 3. Sirt1-deficient BMDMs enhanced cytokine secretion when treated with HDM. (A) Schematic map of the generation of Sirt1fl/fl-LysMCre mice. (B) Genotyping was performed by PCR using mouse tail genomic DNA. (C, D) Genotyping was assessed in BMDMs from Sirt1fl/fl and Sirt1fl/fl-LysMCre mice using Western blot and RT-PCR analysis. (E–H) BMDMs were treated with HDM at 100 μg/ml for 24 h to measure the levels of Cxcl2, Il-1β, Il-6, and Tnf-α. Data are presented as Mean ± SEM of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001.