Figure 5. Sirt1-deficient exacerbates inflammatory cytokines production following allergen exposure. Sirt1fl/fl-LysMCre mice and Sirt1fl/fl littermates were established allergic asthma model (n = 5-8 mice per group per study). Lungs were isolated 1 day after the last challenge. (A–C) Expression of the mRNA levels of inflammatory cytokines in the lung homogenate were analyzed by RT-PCR; (D–F) Protein levels of inflammatory cytokines in the lung homogenate were measured by ELISA. Data are representative of three independent experiments with similar results. Data are presented as Mean ± SEM of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001.