Research Paper Volume 13, Issue 11 pp 15479—15490

Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a mouse model of allergic asthma

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Figure 5.

Sirt1-deficient exacerbates inflammatory cytokines production following allergen exposure. Sirt1fl/fl-LysMCre mice and Sirt1fl/fl littermates were established allergic asthma model (n = 5-8 mice per group per study). Lungs were isolated 1 day after the last challenge. (AC) Expression of the mRNA levels of inflammatory cytokines in the lung homogenate were analyzed by RT-PCR; (DF) Protein levels of inflammatory cytokines in the lung homogenate were measured by ELISA. Data are representative of three independent experiments with similar results. Data are presented as Mean ± SEM of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001.