Research Paper Volume 13, Issue 11 pp 15479—15490

Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a mouse model of allergic asthma

SIRT1 suppresses airway inflammation partially through inhibition of ERK/p38 MAPK pathway activation. (A, B) Sirt1fl/fl and Sirt1fl/fl-LysMCre BMDMs were treated with HDM, the expression of p-p38 and p-ERK was assessed by using Western blot analysis; (C–E) p38 inhibitor SB203580 and ERK inhibitor U0126 were added in HDM-exposed BMDMs and BMDMs were collected after 24 h. The levels of Cxcl2, Il-1β, and Il6 were analyzed by RT-PCR. Data are representative of three independent studies. Results are expressed as mean ± SEM. *P

Figure 6. SIRT1 suppresses airway inflammation partially through inhibition of ERK/p38 MAPK pathway activation. (A, B) Sirt1fl/fl and Sirt1fl/fl-LysMCre BMDMs were treated with HDM, the expression of p-p38 and p-ERK was assessed by using Western blot analysis; (CE) p38 inhibitor SB203580 and ERK inhibitor U0126 were added in HDM-exposed BMDMs and BMDMs were collected after 24 h. The levels of Cxcl2, Il-1β, and Il6 were analyzed by RT-PCR. Data are representative of three independent studies. Results are expressed as mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001.