Figure 2. Rg3-CNT decreases PD-L1 expression in TNBC cells. (A–E) The MDA-MB-231 and BT-549 cells were treated with Rg3 (60 μg/ml) or Rg3-CNT (60 μg/ml). (A, B) The expression of PD-L1 was analyzed by fluorescence-activated cell sorting (FACS) in the cells. (C, D) The mRNA expression of BRD4 was measured by qPCR in the cells. (E, F) The protein expression of BRD4 was tested by Western blot analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: ns no significance, ** P < 0.01.