Figure 5. Rg3-CNT reduces the PD-1/PD-L1 axis in a T cell/triple-negative TNBC cell co-culture system. (A, B) MDA-MB-231 or BT-549 cells alone or co-cultured with unstimulated PBMCs or activated T cells obtained from human normal blood donors at a ratio of 1:10 (tumor cells: T cells) were analyzed for PD-L1 expression. (C, D) MDA-MB-231 and BT-549 cells were cultured with the generated conditioned media prior to PD-L1 expression analysis. (E, F) The cell viability was measured by the MTT assays in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: ns no significance, ** P < 0.01.