Research Paper Volume 13, Issue 12 pp 16062—16071

PirB functions as an intrinsic suppressor in hippocampal neural stem cells

PirB increases with age in hippocampal neural stem cells. (A) Expression pattern of PirB human ortholog LilrB2 in the central nervous system as analyzed by a web source dataset: <a href="http://www.alzdata.org/Normalized

Figure 1. PirB increases with age in hippocampal neural stem cells. (A) Expression pattern of PirB human ortholog LilrB2 in the central nervous system as analyzed by a web source dataset: http://www.alzdata.org/Normalized_differential.php. (B) qRT-PCR of PirB mRNA expression in NSCs isolated from mice of different ages. (CE) Dentate gyrus (DG) volume quantified over time using three-dimensional volumetric reconstructions following Nissl staining and shown to be enlarged in PirB-depletion mice (PirB KO) compared to wild type (WT) at different ages. n ≥ 3. Red lines and circles: thickness of the granular layer and the size of single cells, respectively. (E) Neuronal cell number increased upon PirB depletion based on the thicker granular layer and unaffected cell density or size. Data is shown the percentage of control. Means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; ns = no significant difference by the t-test.