Figure 2. PirB knockout promotes NSC stemness maintenance. (A) PirB knockout (KO) was verified by western blot. Arrow: PirB molecular weight. (B–C) Neurospheres from wild-type and PirB-depleted animals indicate that PirB-deficient progenitors show an increased self-renewal capacity. (D) PirB depletion promoted cell proliferation. (E–F) PirB deficiency increased DNA synthesis in NSCs as shown by BrdU incorporation. Scale bar: 50 μm. (G) PirB knockout promoted cell cycle transition in NSCs, as measured by propidium iodide staining and flow cytometry. (H) 3,3’-diaminobenzidine (DAB) staining for PCNA shows the hippocampal dentate gyrus cell proliferation in 2- and 6-month-old mice, respectively. n ≥ 3. Means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; ns = no significant difference by the t-test.