Figure 4. Hyperglycemia inhibited MEK5 phosphorylation and MEK5/ERK5 pathway activation. (A) Western blot analysis of MEK5/ERK5 protein phosphorylation in the hippocampus 48 h after TBI or sham surgery. Bar graphs illustrate densitometric analyses of (B) p-MEK5/MEK5 and (C) p-ERK5/ERK5. All data are presented as the mean ± standard error (n = 5 per group). Statistical significance was determined using one-way ANOVA followed by post-hoc Bonferroni correction. #P < 0.05 or ##P < 0.01 vs. the Sham group; *P < 0.05 or **P < 0.01 vs. the TBI group.