Figure 6. HG reduced cell viability and aggravated neuroinflammation by inhibiting the MEK5/ERK5 pathway. (A) Cellular viability was detected using a CCK-8 assay. The transfection efficiency was confirmed using (B) Western blot analysis and (C) qRT-PCR. **P < 0.01 vs. the pcDNA3.1 vector group. Bar graphs illustrate the relative mRNA levels of (D) IL-1β, (E) TNF-α, (F) IL-10 and (G) TGF-β. β-actin was used as the qRT-PCR control. Data are presented as the mean ± standard deviation (n = 5 per group). Statistical significance was determined using one-way ANOVA followed by post-hoc Bonferroni correction. #P < 0.05 or ##P < 0.01 vs. the Control group; *P < 0.05 or **P < 0.01 vs. the TBI group; &P < 0.05 or &&P < 0.01 vs. the TBI+HG group.